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Effect of royal jelly (RJ) on normal cells. <t>L929</t> and PBMC were used as models of normal cells. The MTT assay indicated that RJ has no cytotoxic effect on L929 and PBMC cells (A). Analysis of apoptosis using Annexin-V/PI staining and flow cytometry revealed that RJ has no significant apoptotic effect on normal cells (B). Hemolytic activity evolution suggested that the hemolysis rate decreases when RJ concentration increases (C). The values are given as the mean SD of three independent experiments. * P ≤0.05 represents significant statistical changes from the control sample
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Effect of royal jelly (RJ) on normal cells. L929 and PBMC were used as models of normal cells. The MTT assay indicated that RJ has no cytotoxic effect on L929 and PBMC cells (A). Analysis of apoptosis using Annexin-V/PI staining and flow cytometry revealed that RJ has no significant apoptotic effect on normal cells (B). Hemolytic activity evolution suggested that the hemolysis rate decreases when RJ concentration increases (C). The values are given as the mean SD of three independent experiments. * P ≤0.05 represents significant statistical changes from the control sample

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL

doi: 10.22038/IJBMS.2024.76261.16498

Figure Lengend Snippet: Effect of royal jelly (RJ) on normal cells. L929 and PBMC were used as models of normal cells. The MTT assay indicated that RJ has no cytotoxic effect on L929 and PBMC cells (A). Analysis of apoptosis using Annexin-V/PI staining and flow cytometry revealed that RJ has no significant apoptotic effect on normal cells (B). Hemolytic activity evolution suggested that the hemolysis rate decreases when RJ concentration increases (C). The values are given as the mean SD of three independent experiments. * P ≤0.05 represents significant statistical changes from the control sample

Article Snippet: Cell culture and treatment Acute Lymphoblastic Leukemia cell line (Nalm-6) and normal mouse fibroblast cell line (L929) were purchased from the Pasteur Institute collection (Tehran, Iran).

Techniques: MTT Assay, Staining, Flow Cytometry, Activity Assay, Concentration Assay, Control